actin polymerization kit Search Results


fluorescence actin polymerization kit  (Cytoskeleton Inc)
95
Cytoskeleton Inc fluorescence actin polymerization kit
Effects of C1 and C2 on actin <t>polymerization</t> with or without Pfn1 in vitro. A, Coomassie staining of an SDS-PAGE showing the purity of actin and GST–Pfn1 used in the pyrene–actin assay. B and C, pyrene–actin polymerization assay curves for the indicated experimental conditions (B, C1; C, C2) recorded for 30 min after addition of the polymerization buffer. Each time point represents the mean ± S.D. values of the <t>fluorescence</t> intensity of polymerized pyrene–actin relative to the maximum fluorescence intensity for the actin alone condition (data are summarized from n = 3 experiments). The insets show the chemical structures of the two compounds. The numbers in parentheses indicate relative concentrations of actin, GST–Pfn1, and the compounds. The actual concentrations of actin and Pfn1 were 10 μm and 40 μm, respectively. C1 or C2 was added either at a 50 μm (Pfn1:compound = 1:1.25) or 100 μm (Pfn1:compound = 1:2.5) concentration.
Fluorescence Actin Polymerization Kit, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/actin+polymerization+kit/pmc05818201-352-17-21?v=Cytoskeleton+Inc
Average 95 stars, based on 1 article reviews
fluorescence actin polymerization kit - by Bioz Stars, 2026-07
95/100 stars
  Buy from Supplier
actin nucleation and polymerization antibody sampler kit  (Cell Signaling Technology Inc)
N/A
Actin nucleation is the process of forming new actin filaments and is necessary to stimulate actin polymerization. Actin polymerization is vital for cell motility, cell division, and cell adhesion. Rac and Cdc42, members of the
   Buy from Supplier

Image Search Results


Effects of C1 and C2 on actin polymerization with or without Pfn1 in vitro. A, Coomassie staining of an SDS-PAGE showing the purity of actin and GST–Pfn1 used in the pyrene–actin assay. B and C, pyrene–actin polymerization assay curves for the indicated experimental conditions (B, C1; C, C2) recorded for 30 min after addition of the polymerization buffer. Each time point represents the mean ± S.D. values of the fluorescence intensity of polymerized pyrene–actin relative to the maximum fluorescence intensity for the actin alone condition (data are summarized from n = 3 experiments). The insets show the chemical structures of the two compounds. The numbers in parentheses indicate relative concentrations of actin, GST–Pfn1, and the compounds. The actual concentrations of actin and Pfn1 were 10 μm and 40 μm, respectively. C1 or C2 was added either at a 50 μm (Pfn1:compound = 1:1.25) or 100 μm (Pfn1:compound = 1:2.5) concentration.

Journal: The Journal of Biological Chemistry

Article Title: Structure-based virtual screening identifies a small-molecule inhibitor of the profilin 1–actin interaction

doi: 10.1074/jbc.M117.809137

Figure Lengend Snippet: Effects of C1 and C2 on actin polymerization with or without Pfn1 in vitro. A, Coomassie staining of an SDS-PAGE showing the purity of actin and GST–Pfn1 used in the pyrene–actin assay. B and C, pyrene–actin polymerization assay curves for the indicated experimental conditions (B, C1; C, C2) recorded for 30 min after addition of the polymerization buffer. Each time point represents the mean ± S.D. values of the fluorescence intensity of polymerized pyrene–actin relative to the maximum fluorescence intensity for the actin alone condition (data are summarized from n = 3 experiments). The insets show the chemical structures of the two compounds. The numbers in parentheses indicate relative concentrations of actin, GST–Pfn1, and the compounds. The actual concentrations of actin and Pfn1 were 10 μm and 40 μm, respectively. C1 or C2 was added either at a 50 μm (Pfn1:compound = 1:1.25) or 100 μm (Pfn1:compound = 1:2.5) concentration.

Article Snippet: Actin polymerization with or without GST–Pfn1 in the presence of DMSO or compound was performed using a fluorescence actin polymerization kit (Cytoskeleton, BK003) according to the protocol of the manufacturer, downscaled to a 96-well format with a 10:1 ratio (9 μ m unlabeled and 1 μ m pyrene actin) between unlabeled and pyrene actin.

Techniques: In Vitro, Staining, SDS Page, Pyrene Actin Assay, Polymerization Assay, Fluorescence, Concentration Assay